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Friday, July 31, 2020 | History

2 edition of Factors involved in deoxyribonucleic acid ligation in Escherichia coli cells found in the catalog.

Factors involved in deoxyribonucleic acid ligation in Escherichia coli cells

Juhani SyvaМ€oja

Factors involved in deoxyribonucleic acid ligation in Escherichia coli cells

by Juhani SyvaМ€oja

  • 397 Want to read
  • 36 Currently reading

Published by University of Oulu in Oulu .
Written in English

    Subjects:
  • DNA ligases.,
  • Adenosine triphosphate -- Metabolism.,
  • Escherichia coli -- Genetics.,
  • DNA repair.,
  • DNA -- Synthesis.

  • Edition Notes

    StatementJuhani Syväoja.
    SeriesActa Universitatis Ouluensis., no. 184
    Classifications
    LC ClassificationsQP619.D53 S98 1987
    The Physical Object
    Pagination43 p. :
    Number of Pages43
    ID Numbers
    Open LibraryOL2461935M
    ISBN 109514223608
    LC Control Number87168376

    DEOXYRIBONUCLEIC ACID-ENVELOPE COMPLEXES FROM ESCHERICHIA COLI A Complex-Specific Protein and Its Possible Function for the Stability of the Complex HANS-G. HEIDRICH and WILLIAM L. OLSEN From the Department Hannig and Department Hofschneider, Max Planck Institut f'tir Biochemie, Martinsried b. M~inchen, West Germany. Dr. mass, number of viable cells, and deoxyribonucleic acid [DNA], RNA, and tase activity in crude extracts of Escherichia coli, but Rosenkranz and Jacobs (41) have dine pools were labeled bygrowinglog-phase cells of E. coli B/5 in the presence of Mmoleof "4C-uracil ( x counts per min per Mmole; mCi/.

    Escherichia coli value-added products systems biology synthetic biology cell factory network reconstruction metabolic capability spider silk protein polyhydroxyalkanoate polylactic acid propanediol diamine putrescine cadaverine ethanol butanol isobutanol biodiesel hydrogen farnesol biofuel amino acids valine threonine isoprenoids artemisinin. Deoxyribonucleic Acid Polymerase III of Escherichia coli PURIFICATION AND PROPERTIES* (Received for publication, July 8, ) DENNIS M. LIVINGSTON,$ DAVID C. HINKLE,~ AND CHARLES C. RICHARDSON~ From the Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts SUMMARY.

    acid-precipitable materials. When E. coli HFS grown to 5 x to 5 x 10' cells per ml was sus-pended in TPG-CA medium containing 2 Mg of thy-mine per ml, [3H]thymine was added at a final con-centration of to 5,Ci/ml. Aportion was taken out and 10% ice-cold trichloroacetic acid was added. The mixture was allowed to stand on ice for at. Gut microbiota composition influences the balance between human health and disease. Increasing evidence suggests the involvement of microbial factors in regulating cancer development, progression, and therapeutic response. Distinct microbial species have been implicated in modulating gut environment and architecture that affects cancer therapy outcomes.


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Factors involved in deoxyribonucleic acid ligation in Escherichia coli cells by Juhani SyvaМ€oja Download PDF EPUB FB2

Abstract. The experiments presented in this paper suggest that the shift observed in sedimentation of deoxyribonucleic acid from cells of Escherichia coli subjected to amino acid starvation is related to inhibition of ribonucleic acid synthesis rather than to its release from Cited by: 1.

Author(s): Syväoja,Juhani Title(s): Factors involved in deoxyribonucleic acid ligation in Escherichia coli cells/ Juhani Syväoja. Country of Publication: Finland Publisher: Oulu: University of Oulu, Description: 1 v.

(various pagings): ill. Boyle JV, Goss WA, Cook TM. Induction of excessive deoxyribonucleic acid synthesis in Escherichia coli by nalidixic acid.

J Bacteriol. Nov; 94 (5)– [PMC free article] Boyle JV, Cook TM, Goss WA. Mechanism of action of nalidixic acid on Escherichia coli. Cell-free studies. J Bacteriol. Jan; 97 (1)–Cited by: Marinus MG, Adelberg EA. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K J Bacteriol.

Dec; (3)– [PMC free article] Matsubara K. Properties of sex factor and related episomes isolated from purified Escherichia coli zygote by: Sigma-Aldrich offers a number of Deoxyribonucleic acid sodium salt from Escherichia coli strain B products.

View information & documentation regarding Deoxyribonucleic acid sodium salt from Escherichia coli strain B, including CAS, MSDS & more. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol.No. 19, Issue of Octo pp. Printed in U.S.A. The Deoxyribonucleic Acid Modification and Restriction Enzymes of Escherichia coli B I.

PURIFICATION, SUBUNIT STRUCTURE, AND CATALYTIC PROPERTIES OF THE MODIFICATION. Deoxyribonucleic acid (/ d iː ˈ ɒ k s ɪ ˌ r aɪ b oʊ nj uː ˌ k l iː ɪ k,-ˌ k l eɪ-/ (); DNA) is a molecule composed of two polynucleotide chains that coil around each other to form a double helix carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many and ribonucleic acid (RNA) are nucleic acids.

Step la: Preparation of Extract-Escherichia coli B frozen cell paste ( g) was suspended in 13 liters of 50 mM Tris-HCl (pH ), sucrose with the aid of a Waring Blendor. The suspension was lysed by three consecutive passes through a Manton-Gaulin pressure cell at p.s.i.

at 4”. The lysate. 1. Definition. Diarrhoeagenic Escherichia coli (DEC), as a pathogroup, requires special attention since it is responsible for up to 30–40% of acute diarrhoea episodes in children from developing ichia coli (E.

coli) is also recognized as an important pathogen in the industrialized currently known E. coli categories associated with intestinal diseases are.

1. Introduction. Escherichia coli is a widespread commensal inhabitant of the intestinal tract of animals, including humans, and is thought to play a beneficial role in the host.

However, a variety of E. coli pathotypes capable of causing intestinal infections have also evolved. These include enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enteroinvasive E coli (EIEC. This enzyme is normally present at only very low levels in species possessing it (for example, 10 to 15 molecules per cell in Escherichia coli K) (Harm et al., ).

A number of features of its role and regulation might be clarified by cloning the gene on a suitable vehicle, and amplifying the amounts present in viable cells.

Abstract. A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia deoxyribonucleic acid (DNA) was specifically labeled in a female cell after conjugation.

Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for: • PCR reactions of genomic DNA of Gardnerella vaginalis • nick translation and radiolabeling of dNTP • generating standard curve for 16S rDNA quantification • transfecting neutrophils •.

The Nus factors—NusA, NusB, NusE, and NusG—area set of well-conserved proteins in bacteria and are involved in transcription elongation, termination, antitermination, and translation processes.

Originally, Escherichia coli host mutations defective for supporting bacteriophage λ N-mediated antitermination were mapped to the nusA (nusA1), nusB (nusB5, nusB), and nusE (nusE71). Conjugalreplication ofR deoxyribonucleic acid (DNA)andtheconcomi- acid (DNA) from donor to recipient cells of Escherichia coli.

They proposed that contact between donor and recipient cells triggers the shownto promotecuringofF' factors in E. coli and to inhibit growth of integratively sup-pressed Hfr strains (2). deoxyribonucleic acid (DNA)-membranous complexes from bacteria have been reported. For genetic reasons most of these studies have involved Escherichia coli.

Thesurfacestructure of gram-negative bacteria such as E. coli is a complex multilayered envelope composedofan inner plasma membrane (referred to in this paper as membrane) andthe cell wall. In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme.

It is an essential laboratory procedure in the molecular cloning of DNA whereby DNA fragments are joined together to create recombinant DNA molecules, such as when a foreign DNA fragment is inserted into a ends of DNA fragments are joined together by the formation of.

A crystal structure of a fragment of the Escherichia coli enzyme [59] supports the mechanism in Figures and In the case of a topoisomerase type IB, nucleophilic attack by the tyrosine residue breaks the DNA strand to generate a phosphodiester link between the tyrosine and the 3′-phosphate, releasing a 5′-hydroxyl end (Scheme [Show full abstract] presented which indicates that thse sensitized deoxyribonucleic acid lesions contribute to lethality for Escherichia coli irradiated with nm ultraviolet light in.

" any of a class of single-stranded molecules transcribed from DNA in the cell nucleus or in the mitochondrion or chloroplast, containing along the strand a linear sequence of nucleotide bases that is complementary to the DNA strand from which it is transcribed: the composition of the RNA molecule is identical with that of DNA except for the substitution of the sugar ribose for deoxyribose and.

Different toxicity of Escherichia coli and Agrobacterium tumefaciens against tobacco BY2 cells Article in Nucleic Acids Symposium Series 44(44) February with 18 Reads.Mutation Affecting Resistance of Escherichia coli K12 to Nalidixic Acid Article (PDF Available) in Journal of general microbiology (9) October with 94 Reads How we measure 'reads'.Cloning and expression in Escherichia coli of genes involved in the lysine pathway of Brevibacterium lactofermentum Article (PDF Available) in Journal of Bacteriology (1) November.